CD28 antibody

Catalog No. ABIN1177271

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS and IP. It is suitable to detect CD28 in samples from Rat. There are 3+ publications available.

Quick Overview for CD28 antibody (ABIN1177271)

Target: CD28

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: JJ316

Product Details anti-CD28 Antibody

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Rat Cd28-transfected BALB/c mouse A20J B lymphoma

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD28 antibody (ABIN1177271)

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Mitnacht, Tacke, Hünig et al.: "Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, ..." in: European journal of immunology, Vol. 25, Issue 2, pp. 328-32, (1995) (PubMed).

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: The JJ316 antibody reacts with CD28 on virtually all T lymphocytes bearing alphabetaT-cell receptors (TCR), on most gammadeltaTCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of alphabetaTCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells, its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ316 enhances the proliferative responses and IL-2 production by T cells activated by anti-aß-TCR mAb R73 (Cat. no. 554910). Furthermore, at high concentrations, cross-linked JJ316 mAb stimulates resting CD4+ and CD8+T cells to proliferate, secrete IL-2, express activation markers, and gain CTL activity in the absence of signaling through the TCR. In vivo administration of mAb JJ316 results in blastogenic transformation of peripheral CD4+ cells and dramatic lymphocytosis. The alternate anti-rat CD28 mAb JJ319 (Cat. no. 554993) cannot directly activate T cells, although it has in vitro costimulatory activity.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response